NEET Biology / AI question bank / NCERT Class 12 / Biotechnology and Its Applications
Biotechnology: Principles and Processes: NEET questions with solutions
This chapter covers restriction enzymes, vectors, gel electrophoresis, PCR and bioreactors. Marks are lost on direction of DNA movement, selection of recombinants, the roles of enzymes used to open cells and the names of PCR steps.
Also called: recombinant DNA technology · genetic engineering · PCR · restriction enzymes
Questions
10 questions / answers after you pick
- Q1In the name EcoRI of a restriction enzyme, what does the letter R represent?
Restriction enzymesEasySingle correct
- Q2In agarose gel electrophoresis of a DNA digest, towards which electrode do the fragments move, and which fragments travel farthest from the…
Gel electrophoresisEasySingle correct
- Q3Which feature of the DNA polymerase used in PCR makes it suitable, and from which organism is it isolated?
Polymerase chain reactionEasySingle correct
- Q4Two statements about restriction enzymes are given. Select the correct option.
Restriction enzymesMediumStatement based
- Q5Read the Assertion and the Reason about selecting recombinants with a plasmid vector in which foreign DNA is inserted at the BamH I site…
Cloning vectorsMediumAssertion–Reason
- Q6Match each agent used while isolating DNA in Column I with its specific use in Column II.
Isolation of the genetic materialMediumMatch the following
- Q7In a vector with a coloured-colony selection system based on β-galactosidase, a colourless colony on a chromogenic substrate indicates:
Cloning vectorsMediumSingle correct
- Q8Which sequence correctly describes making bacterial cells take up a recombinant plasmid by the usual chemical method?
Introducing DNA into the hostMediumSingle correct
- Q9Each PCR cycle doubles the number of copies of the target DNA. Starting from a single copy, about how many copies exist after 30 cycles?
Polymerase chain reactionHardSingle correct
- Q10A recognition site is palindromic if the 5'→3' sequence of one strand equals the 5'→3' sequence of its complementary strand. Which…
Restriction enzymesHardSingle correct
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